streptavidin coated sensor chip sa  (Biacore)

Journal: Biosensors

Article Title: Exploring the Interaction of Biotinylated FcGamma RI and IgG1 Monoclonal Antibodies on Streptavidin-Coated Plasmonic Sensor Chips for Label-Free VEGF Detection

doi: 10.3390/bios14120634

Figure Lengend Snippet: A schematic of the VEGF SPR biosensor setup. Biotinylated FcγRI is immobilized on the streptavidin-coated sensor surface. The mAb is captured by the immobilized FcγRI through high-affinity interactions with the mAb Fc regions, orienting the mAb Fab regions for the optimal detection and quantification of VEGF. Created in BioRender. Khalid-Salako, F. (2024) https://BioRender.com/g42b753 .

Article Snippet: The Sensor chip SA with streptavidin pre-coated on a carboxymethylated dextran matrix surface functionalization was inserted and docked on the Biacore T200 instrument (Cytiva, Marlborough, MA, USA), which was then primed following the manufacturer’s instructions.

Techniques:

Journal: Biosensors

Article Title: Exploring the Interaction of Biotinylated FcGamma RI and IgG1 Monoclonal Antibodies on Streptavidin-Coated Plasmonic Sensor Chips for Label-Free VEGF Detection

doi: 10.3390/bios14120634

Figure Lengend Snippet: Sensorgram of biotinylated−FcγRI immobilization on streptavidin-coated SPR sensor chip.

Article Snippet: The Sensor chip SA with streptavidin pre-coated on a carboxymethylated dextran matrix surface functionalization was inserted and docked on the Biacore T200 instrument (Cytiva, Marlborough, MA, USA), which was then primed following the manufacturer’s instructions.

Techniques:

Journal: Molecular Therapy. Nucleic Acids

Article Title: CD3 aptamers promote expansion and persistence of tumor-reactive T cells for adoptive T cell therapy in cancer

doi: 10.1016/j.omtn.2024.102198

Figure Lengend Snippet: CD3 aptamers boost and induce T cell activation (A) Two types of CD3 aptamer-containing SLBs were created; one containing biotinylated CD3 aptamers (attached via ABS635P-labeled monovalent streptavidin), pMHC, B7-1, and ICAM-1 (A) to measure the capacity of CD3 aptamers to induce calcium flux in the presence of suboptimal pMHC T cell stimulus, and one without pMHC (B) to study the aptamers’ endogenous activation capacity. (C) Dose-response curves for T cell activation of CD3 aptamers ± suboptimal pMHC stimulus. Apt Ctrl, Apt 1, and Apt 12 were decorated at various ligand densities on SLBs with or without suboptimal pMHC. The percentage of activated cells was determined for each condition and normalized to the positive control (100% T cell activation). SLBs ranked from lowest to highest activation thresholds: Apt 12 + pMHC < Apt 1 + pMHC < Apt 12 < Apt 1 < Apt Ctrl + pMHC < Apt Ctrl. Dose-response curves were analyzed as described in Hellmeier et al. to extract activation thresholds (D and E). For all calcium flux experiments, 13 ligand densities were assayed. n = 13 SLBs. A two-sided Bootstrap ratio test hoc was performed, the extracted values are extracted from a fit, and error bars represent the 95% CI. ∗∗∗ p value < 0.001.

Article Snippet: Biotinylated aptamers were immobilized onto independent channels of streptavidin-coated sensor chips (Bio-Rad).

Techniques: Activation Assay, Labeling, Positive Control